Deletion of the Candida albicans TLO gene family using CRISPR-Cas9 mutagenesis allows characterisation of functional differences in α-, β- and γ- TLO gene function.

Deletion of the Candida albicans TLO gene family using CRISPR-Cas9 mutagenesis allows characterisation of functional differences in α-, β- and γ- TLO gene function.

The Candida albicans genome contains between ten and fifteen distinct TLO genes that all encode a Med2 subunit of Mediator. In order to investigate the biological role of Med2/Tlo in C. albicans we deleted all fourteen TLO genes using CRISPR-Cas9 mutagenesis. ChIP-seq analysis showed that RNAP II lo...

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Bibliographic Details
Main Authors: Jessica Fletcher, James O'Connor-Moneley, Dean Frawley, Peter R Flanagan, Leenah Alaalm, Pilar Menendez-Manjon, Samuel Vega Estevez, Shane Hendricks, Andrew L Woodruff, Alessia Buscaino, Matthew Z Anderson, Derek J Sullivan, Gary P Moran
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-12-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011082&type=printable

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https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1011082&type=printable

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