Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages
We report improved automated open-source methodology for head-fixed mesoscale cortical imaging and/or behavioral training of home cage mice using Raspberry Pi-based hardware. Staged partial and probabilistic restraint allows mice to adjust to self-initiated headfixation over 3 weeks’ time with ~50%...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/55964 |
| _version_ | 1811201248758595584 |
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| author | Timothy H Murphy Nicholas J Michelson Jamie D Boyd Tony Fong Luis A Bolanos David Bierbrauer Teri Siu Matilde Balbi Federico Bolanos Matthieu Vanni Jeff M LeDue |
| author_facet | Timothy H Murphy Nicholas J Michelson Jamie D Boyd Tony Fong Luis A Bolanos David Bierbrauer Teri Siu Matilde Balbi Federico Bolanos Matthieu Vanni Jeff M LeDue |
| author_sort | Timothy H Murphy |
| collection | DOAJ |
| description | We report improved automated open-source methodology for head-fixed mesoscale cortical imaging and/or behavioral training of home cage mice using Raspberry Pi-based hardware. Staged partial and probabilistic restraint allows mice to adjust to self-initiated headfixation over 3 weeks’ time with ~50% participation rate. We support a cue-based behavioral licking task monitored by a capacitive touch-sensor water spout. While automatically head-fixed, we acquire spontaneous, movement-triggered, or licking task-evoked GCaMP6 cortical signals. An analysis pipeline marked both behavioral events, as well as analyzed brain fluorescence signals as they relate to spontaneous and/or task-evoked behavioral activity. Mice were trained to suppress licking and wait for cues that marked the delivery of water. Correct rewarded go-trials were associated with widespread activation of midline and lateral barrel cortex areas following a vibration cue and delayed frontal and lateral motor cortex activation. Cortical GCaMP signals predicted trial success and correlated strongly with trial-outcome dependent body movements. |
| first_indexed | 2024-04-12T02:18:02Z |
| format | Article |
| id | doaj.art-6888822d4322476dba70dfcb3f8d21c7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:18:02Z |
| publishDate | 2020-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6888822d4322476dba70dfcb3f8d21c72022-12-22T03:52:12ZengeLife Sciences Publications LtdeLife2050-084X2020-05-01910.7554/eLife.55964Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cagesTimothy H Murphy0https://orcid.org/0000-0002-0093-4490Nicholas J Michelson1Jamie D Boyd2Tony Fong3Luis A Bolanos4David Bierbrauer5Teri Siu6Matilde Balbi7Federico Bolanos8Matthieu Vanni9Jeff M LeDue10Department of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaDepartment of Psychiatry, Kinsmen Laboratory of Neurological Research, Vancouver, Canada; Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, CanadaWe report improved automated open-source methodology for head-fixed mesoscale cortical imaging and/or behavioral training of home cage mice using Raspberry Pi-based hardware. Staged partial and probabilistic restraint allows mice to adjust to self-initiated headfixation over 3 weeks’ time with ~50% participation rate. We support a cue-based behavioral licking task monitored by a capacitive touch-sensor water spout. While automatically head-fixed, we acquire spontaneous, movement-triggered, or licking task-evoked GCaMP6 cortical signals. An analysis pipeline marked both behavioral events, as well as analyzed brain fluorescence signals as they relate to spontaneous and/or task-evoked behavioral activity. Mice were trained to suppress licking and wait for cues that marked the delivery of water. Correct rewarded go-trials were associated with widespread activation of midline and lateral barrel cortex areas following a vibration cue and delayed frontal and lateral motor cortex activation. Cortical GCaMP signals predicted trial success and correlated strongly with trial-outcome dependent body movements.https://elifesciences.org/articles/55964cortexhomecageautomationmesoscaleimaging |
| spellingShingle | Timothy H Murphy Nicholas J Michelson Jamie D Boyd Tony Fong Luis A Bolanos David Bierbrauer Teri Siu Matilde Balbi Federico Bolanos Matthieu Vanni Jeff M LeDue Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages eLife cortex homecage automation mesoscale imaging |
| title | Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| title_full | Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| title_fullStr | Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| title_full_unstemmed | Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| title_short | Automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| title_sort | automated task training and longitudinal monitoring of mouse mesoscale cortical circuits using home cages |
| topic | cortex homecage automation mesoscale imaging |
| url | https://elifesciences.org/articles/55964 |
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