Development of cPCR technique for detection and enumeration of Prevotella bryantii

Competitive PCR (cPCR) system for the detection and enumeration of Prevotella bryantii cells in rumen samples was developed. PBB14 primer, specific for P. bryantii was used as the reverse PCR primer and EUB 338 bacterial primer as the forward primer. An internal DNA control, containing primer speci...

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Bibliographic Details
Main Authors: Katarina TEPŠIČ, Gorazd AVGUŠTIN
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 1999-12-01
Series:Acta Agriculturae Slovenica
Subjects:
Online Access:https://journals.uni-lj.si/aas/article/view/15907