Multi-enzyme isothermal rapid amplification assay for the detection of Escherichia coli O157∶H7

Multi-enzyme isothermal rapid amplification assay for the detection of Escherichia coli O157∶H7

ObjectiveA rapid enrichment and detection method for Escherichia coli O157∶H7 was developed by using multienzyme isothermal rapid amplification （MIRA） fluorescence method combined with metal organic frameworks immunomagnetic beads.MethodsUsing rfbE gene as the target， the primers， probes and reactio...

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Bibliographic Details
Main Authors: WANG Shujuan, FAN Yiling, FENG Zhen, JIANG Bo, SONG Minghui, LI Qiongqiong, LIU Hao, QIN Feng, YANG Meicheng
Format: Article
Language:zho
Published: Shanghai Preventive Medicine Association 2022-06-01
Series:Shanghai yufang yixue
Subjects:
multienzyme isothermal rapid amplification
metal-organic framework immunomagnetic bead
rfbe gene
escherichia coli o157∶h7
Online Access:http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.22048

Internet

http://www.sjpm.org.cn/article/doi/10.19428/j.cnki.sjpm.2022.22048

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