A simple and rapid method to measure cholesterol binding to P450s and other proteins
Cholesterol plays an important role in cellular function and membrane compartmentalization and is involved in the interaction with more than a dozen of different proteins. Using three cholesterol-metabolizing cytochrome P450s (P450s 7A1, 46A1, and 11A1), we have developed a rapid and simple assay fo...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2005-07-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752033008X |