A simple and rapid method to measure cholesterol binding to P450s and other proteins

Cholesterol plays an important role in cellular function and membrane compartmentalization and is involved in the interaction with more than a dozen of different proteins. Using three cholesterol-metabolizing cytochrome P450s (P450s 7A1, 46A1, and 11A1), we have developed a rapid and simple assay fo...

Full description

Bibliographic Details
Main Authors: Natalia Mast, Irina A. Pikuleva
Format: Article
Language:English
Published: Elsevier 2005-07-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752033008X