Immunostaining of tumor-infiltrating immune-cell-related markers and cytokines in the tumor fractional radiation model of host PD-L1-deficient mice

Immunostaining of tumor-infiltrating immune-cell-related markers and cytokines in the tumor fractional radiation model of host PD-L1-deficient mice

Summary: In this protocol, we describe how to explore immune function in radiation-induced anti-tumor abscopal effect (ATAE) in PD-L1-deficient (PD-L1−/−) mice. We detail steps for primary and abscopal tumor model establishment in mice, tumor fractional irradiation scheme, and isolation of single ce...

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Bibliographic Details
Main Authors: Xinrui Zhao, Chunlin Shao
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Flow Cytometry/Mass Cytometry
Cancer
Immunology
Model Organisms
Signal Transduction
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007390

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http://www.sciencedirect.com/science/article/pii/S2666166722007390

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