Metabolic labeling and LC-MS/MS-based identification of interleukin-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum

Metabolic labeling and LC-MS/MS-based identification of interleukin-1α-induced secreted proteomes from epithelial cells in the presence or absence of serum

Summary: The unbiased identification of cytokine-induced, secreted proteins from cells cultured in serum-containing medium is challenging. Here, we describe an experimental and bioinformatics workflow to label interleukin-1α-regulated proteins in living cells with the methionine analogue L-homopropa...

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Bibliographic Details
Main Authors: Jasmin Priester, Johanna Meier-Soelch, Hendrik Weiser, Daniel Heylmann, Axel Weber, Uwe Linne, Michael Kracht
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Culture
Flow Cytometry/Mass Cytometry
Immunology
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001533

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http://www.sciencedirect.com/science/article/pii/S2666166723001533

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