Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production
Previously, a designed ankyrin repeat protein, Ank<sup>GAG</sup>1D4, was generated for intracellular targeting of the HIV-1 capsid domain. The efficiency was satisfactory in interfering with the HIV assembly process. Consequently, improved Ank<sup>GAG</sup>1D4 binding affinit...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/10/1437 |
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| author | Kanokporn Sornsuwan Weeraya Thongkhum Thanathat Pamonsupornwichit Tanawan Samleerat Carraway Suthinee Soponpong Supachai Sakkhachornphop Chatchai Tayapiwatana Umpa Yasamut |
| author_facet | Kanokporn Sornsuwan Weeraya Thongkhum Thanathat Pamonsupornwichit Tanawan Samleerat Carraway Suthinee Soponpong Supachai Sakkhachornphop Chatchai Tayapiwatana Umpa Yasamut |
| author_sort | Kanokporn Sornsuwan |
| collection | DOAJ |
| description | Previously, a designed ankyrin repeat protein, Ank<sup>GAG</sup>1D4, was generated for intracellular targeting of the HIV-1 capsid domain. The efficiency was satisfactory in interfering with the HIV assembly process. Consequently, improved Ank<sup>GAG</sup>1D4 binding affinity was introduced by substituting tyrosine (Y) for serine (S) at position 45. However, the intracellular anti-HIV-1 activity of Ank<sup>GAG</sup>1D4-S45Y has not yet been validated. In this study, the performance of Ank<sup>GAG</sup>1D4 and Ank<sup>GAG</sup>1D4-S45Y in inhibiting wild-type HIV-1 and HIV-1 maturation inhibitor-resistant replication in SupT1 cells was evaluated. HIV-1 p24 and viral load assays were used to verify the biological activity of Ank<sup>GAG</sup>1D4 and Ank<sup>GAG</sup>1D4-S45Y as assembly inhibitors. In addition, retardation of syncytium formation in infected SupT1 cells was observed. Of note, the defense mechanism of both ankyrins did not induce the mutation of target amino acids in the capsid domain. The present data show that the potency of Ank<sup>GAG</sup>1D4-S45Y was superior to Ank<sup>GAG</sup>1D4 in interrupting either HIV-1 wild-type or the HIV maturation inhibitor-resistant strain. |
| first_indexed | 2024-03-10T06:42:26Z |
| format | Article |
| id | doaj.art-697c9a20c48f44a9825dd6afac6552aa |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T06:42:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-697c9a20c48f44a9825dd6afac6552aa2023-11-22T17:33:31ZengMDPI AGBiomolecules2218-273X2021-09-011110143710.3390/biom11101437Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny ProductionKanokporn Sornsuwan0Weeraya Thongkhum1Thanathat Pamonsupornwichit2Tanawan Samleerat Carraway3Suthinee Soponpong4Supachai Sakkhachornphop5Chatchai Tayapiwatana6Umpa Yasamut7Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandCenter of Biomolecular Therapy and Diagnostic, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandResearch Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandPreviously, a designed ankyrin repeat protein, Ank<sup>GAG</sup>1D4, was generated for intracellular targeting of the HIV-1 capsid domain. The efficiency was satisfactory in interfering with the HIV assembly process. Consequently, improved Ank<sup>GAG</sup>1D4 binding affinity was introduced by substituting tyrosine (Y) for serine (S) at position 45. However, the intracellular anti-HIV-1 activity of Ank<sup>GAG</sup>1D4-S45Y has not yet been validated. In this study, the performance of Ank<sup>GAG</sup>1D4 and Ank<sup>GAG</sup>1D4-S45Y in inhibiting wild-type HIV-1 and HIV-1 maturation inhibitor-resistant replication in SupT1 cells was evaluated. HIV-1 p24 and viral load assays were used to verify the biological activity of Ank<sup>GAG</sup>1D4 and Ank<sup>GAG</sup>1D4-S45Y as assembly inhibitors. In addition, retardation of syncytium formation in infected SupT1 cells was observed. Of note, the defense mechanism of both ankyrins did not induce the mutation of target amino acids in the capsid domain. The present data show that the potency of Ank<sup>GAG</sup>1D4-S45Y was superior to Ank<sup>GAG</sup>1D4 in interrupting either HIV-1 wild-type or the HIV maturation inhibitor-resistant strain.https://www.mdpi.com/2218-273X/11/10/1437ankyrincapsidHIV-1 assemblyanti-HIV-1 moleculeHIV-1 drug resistance |
| spellingShingle | Kanokporn Sornsuwan Weeraya Thongkhum Thanathat Pamonsupornwichit Tanawan Samleerat Carraway Suthinee Soponpong Supachai Sakkhachornphop Chatchai Tayapiwatana Umpa Yasamut Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production Biomolecules ankyrin capsid HIV-1 assembly anti-HIV-1 molecule HIV-1 drug resistance |
| title | Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production |
| title_full | Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production |
| title_fullStr | Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production |
| title_full_unstemmed | Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production |
| title_short | Performance of Affinity-Improved DARPin Targeting HIV Capsid Domain in Interference of Viral Progeny Production |
| title_sort | performance of affinity improved darpin targeting hiv capsid domain in interference of viral progeny production |
| topic | ankyrin capsid HIV-1 assembly anti-HIV-1 molecule HIV-1 drug resistance |
| url | https://www.mdpi.com/2218-273X/11/10/1437 |
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