Long-Read Isoform Sequencing Reveals a Hidden Complexity of the Transcriptional Landscape of Herpes Simplex Virus Type 1

Long-Read Isoform Sequencing Reveals a Hidden Complexity of the Transcriptional Landscape of Herpes Simplex Virus Type 1

In this study, we used the amplified isoform sequencing technique from Pacific Biosciences to characterize the poly(A)+ fraction of the lytic transcriptome of the herpes simplex virus type 1 (HSV-1). Our analysis detected 34 formerly unidentified protein-coding genes, 10 non-coding RNAs, as well as...

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Bibliographic Details
Main Authors: Dóra Tombácz, Zsolt Csabai, Attila Szűcs, Zsolt Balázs, Norbert Moldován, Donald Sharon, Michael Snyder, Zsolt Boldogkői
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-06-01
Series:Frontiers in Microbiology
Subjects:
human herpesvirus 1
herpes simplex virus type 1
transcriptome
transcriptional interference
full-length sequencing
long-read sequencing
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01079/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.01079/full

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