Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice
Summary: Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000916 |
| _version_ | 1797894665700114432 |
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| author | Hao Chen Jian Liu Guo-Ping Shi Xian Zhang |
| author_facet | Hao Chen Jian Liu Guo-Ping Shi Xian Zhang |
| author_sort | Hao Chen |
| collection | DOAJ |
| description | Summary: Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T07:12:54Z |
| format | Article |
| id | doaj.art-69a1e5a732454de0a97bfc2a768e1eaa |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T07:12:54Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-69a1e5a732454de0a97bfc2a768e1eaa2023-02-26T04:28:16ZengElsevierSTAR Protocols2666-16672023-03-0141102133Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic miceHao Chen0Jian Liu1Guo-Ping Shi2Xian Zhang3School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China; Engineering Research Center of Bioprocess, Ministry of Education, Hefei University of Technology, Hefei, Anhui 230009, ChinaDepartment of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USASchool of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China; Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000916Cell BiologyCell IsolationFlow Cytometry/Mass CytometryDevelopmental BiologyHealth SciencesMetabolism |
| spellingShingle | Hao Chen Jian Liu Guo-Ping Shi Xian Zhang Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice STAR Protocols Cell Biology Cell Isolation Flow Cytometry/Mass Cytometry Developmental Biology Health Sciences Metabolism |
| title | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| title_full | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| title_fullStr | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| title_full_unstemmed | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| title_short | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| title_sort | protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
| topic | Cell Biology Cell Isolation Flow Cytometry/Mass Cytometry Developmental Biology Health Sciences Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000916 |
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