Development of a Sensitive and Specific Novel qPCR Assay for Simultaneous Detection and Differentiation of Mucormycosis and Aspergillosis by Melting Curve Analysis

Development of a Sensitive and Specific Novel qPCR Assay for Simultaneous Detection and Differentiation of Mucormycosis and Aspergillosis by Melting Curve Analysis

Molecular diagnostic assays can expedite the diagnosis of fungal infections, and subsequently help in early interventions and appropriate management of patients. The aim of this study was to develop a single set of primers for a real-time quantitative polymerase chain reaction (qPCR) assay to detect...

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Bibliographic Details
Main Authors: Mragnayani Pandey, Immaculata Xess, Janya Sachdev, Usha Yadav, Gagandeep Singh, Dibyabhaba Pradhan, Ashit Bhushan Xess, Bhaskar Rana, Lalit Dar, Sameer Bakhshi, Rachna Seth, Manoranjan Mahapatra, Viveka P. Jyotsna, Arun Kumar Jain, Rakesh Kumar, Reshu Agarwal, Prashant Mani
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Fungal Biology
Subjects:
invasive mucormycosis
invasive aspergillosis
real-time PCR
melting curve analysis
invasive fusariosis
Online Access:https://www.frontiersin.org/articles/10.3389/ffunb.2021.800898/full

Internet

https://www.frontiersin.org/articles/10.3389/ffunb.2021.800898/full

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