Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes
Many host cellular signaling pathways were activated and exploited by virus infection for more efficient replication. The PI3K/Akt pathway has recently attracted considerable interest due to its role in regulating virus replication. This study demonstrated for the first time that the mammalian reovi...
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Frontiers Media S.A.
2015-09-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00886/full |
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author | Jin eTian Xiaozhan eZhang Hongxia eWu Chunguo eLiu Zhijie eLi Xiaoliang eHu Shuo eSu Linfa eWang Liandong eQu |
author_facet | Jin eTian Xiaozhan eZhang Hongxia eWu Chunguo eLiu Zhijie eLi Xiaoliang eHu Shuo eSu Linfa eWang Liandong eQu |
author_sort | Jin eTian |
collection | DOAJ |
description | Many host cellular signaling pathways were activated and exploited by virus infection for more efficient replication. The PI3K/Akt pathway has recently attracted considerable interest due to its role in regulating virus replication. This study demonstrated for the first time that the mammalian reovirus strains Masked Palm Civet/China/2004 (MPC/04) and Bat/China/2003 (B/03) can induce transient activation of the PI3K/Akt pathway early in infection in vitro. When UV-treated, both viruses activated PI3K/Akt signaling, indicating that the virus/receptor interaction was sufficient to activate PI3K/Akt. Reovirus virions can use both clathrin- and caveolae-mediated endocytosis, but only chlorpromazine, a specific inhibitor of clathrin-mediated endocytosis, or siRNA targeting clathrin suppressed Akt phosphorylation. We also identified the upstream molecules of the PI3K pathway. Virus infection induced phosphorylation of focal adhesion kinase (FAK) but not Gab1, and blockage of FAK phosphorylation suppressed Akt phosphorylation. Blockage of PI3K/Akt activation increased virus RNA synthesis and viral yield. We also found that reovirus infection activated the IFN-stimulated response element (ISRE) in an interferon-independent manner and up-regulated IFN-stimulated genes (ISGs) via the PI3K/Akt/EMSY pathway. Suppression of PI3K/Akt activation impaired the induction of ISRE and down-regulated the expression of ISGs. Overexpression of ISG15 and Viperin inhibited virus replication, and knockdown of either enhanced virus replication. Collectively, these results demonstrate that PI3K/Akt activated by mammalian reovirus serves as a pathway for sensing and then inhibiting virus replication/infection. |
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language | English |
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publishDate | 2015-09-01 |
publisher | Frontiers Media S.A. |
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spelling | doaj.art-69bb8c588fee410d90a67cfbe287efee2022-12-22T03:19:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-09-01610.3389/fmicb.2015.00886159244Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated GenesJin eTian0Xiaozhan eZhang1Hongxia eWu2Chunguo eLiu3Zhijie eLi4Xiaoliang eHu5Shuo eSu6Linfa eWang7Liandong eQu8Chinese Academy of Agricultural SciencesChinese Academy of Agricultural SciencesChinese Academy of Agricultural SciencesChinese Academy of Agricultural SciencesChinese Academy of Agricultural SciencesChinese Academy of Agricultural SciencesSouth China Agricultural UniversityDuke-NUS Graduate Medical SchoolChinese Academy of Agricultural SciencesMany host cellular signaling pathways were activated and exploited by virus infection for more efficient replication. The PI3K/Akt pathway has recently attracted considerable interest due to its role in regulating virus replication. This study demonstrated for the first time that the mammalian reovirus strains Masked Palm Civet/China/2004 (MPC/04) and Bat/China/2003 (B/03) can induce transient activation of the PI3K/Akt pathway early in infection in vitro. When UV-treated, both viruses activated PI3K/Akt signaling, indicating that the virus/receptor interaction was sufficient to activate PI3K/Akt. Reovirus virions can use both clathrin- and caveolae-mediated endocytosis, but only chlorpromazine, a specific inhibitor of clathrin-mediated endocytosis, or siRNA targeting clathrin suppressed Akt phosphorylation. We also identified the upstream molecules of the PI3K pathway. Virus infection induced phosphorylation of focal adhesion kinase (FAK) but not Gab1, and blockage of FAK phosphorylation suppressed Akt phosphorylation. Blockage of PI3K/Akt activation increased virus RNA synthesis and viral yield. We also found that reovirus infection activated the IFN-stimulated response element (ISRE) in an interferon-independent manner and up-regulated IFN-stimulated genes (ISGs) via the PI3K/Akt/EMSY pathway. Suppression of PI3K/Akt activation impaired the induction of ISRE and down-regulated the expression of ISGs. Overexpression of ISG15 and Viperin inhibited virus replication, and knockdown of either enhanced virus replication. Collectively, these results demonstrate that PI3K/Akt activated by mammalian reovirus serves as a pathway for sensing and then inhibiting virus replication/infection.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00886/fullEndocytosisreovirusPI3K/AKTIFN-stimulated genesenhancing viral replication |
spellingShingle | Jin eTian Xiaozhan eZhang Hongxia eWu Chunguo eLiu Zhijie eLi Xiaoliang eHu Shuo eSu Linfa eWang Liandong eQu Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes Frontiers in Microbiology Endocytosis reovirus PI3K/AKT IFN-stimulated genes enhancing viral replication |
title | Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes |
title_full | Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes |
title_fullStr | Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes |
title_full_unstemmed | Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes |
title_short | Blocking the PI3K/AKT Pathway Enhances Mammalian Reovirus Replication by Repressing IFN-stimulated Genes |
title_sort | blocking the pi3k akt pathway enhances mammalian reovirus replication by repressing ifn stimulated genes |
topic | Endocytosis reovirus PI3K/AKT IFN-stimulated genes enhancing viral replication |
url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00886/full |
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