Detection of Staphylococcus aureus in food by droplet digital polymerase chain reaction

ObjectiveTo establish a new quantitative method for detection of Staphylococcus aureus in food by droplet digital polymerase chain reaction (ddPCR).MethodsA pair of primers and probe for both qPCR and ddPCR was selected according to the single copy gene of nuc in Staphylococcus aureus. The specifici...

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Bibliographic Details
Main Authors: LI Dan, XU Leirui, WEI Haiyan, WEI Yongxin, MA Dan, WANG Qi, ZHANG Ximeng, ZHANG Feng, ZENG Jing
Format: Article
Language:zho
Published: The Editorial Office of Chinese Journal of Food Hygiene 2021-05-01
Series:Zhongguo shipin weisheng zazhi
Subjects:
Online Access:http://www.zgspws.com/zgspwszz/article/abstract/20210308?st=article_issue