Rapid and precise genome engineering in a naturally short-lived vertebrate
The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here, we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluoresce...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2023-05-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/80639 |