Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics

Fiber-Optic Fluoroimmunoassay System with a Flow-Through Cell for Rapid On-Site Determination of Escherichia coli O157:H7 by Monitoring Fluorescence Dynamics

Dynamic fluoroimmunoassay with a flow-through system using optical fiber probes consisting of polystyrene was developed and applied to a quantitative detection of E. coli O157:H7. The system measures E. coli as fluorescence of sandwich-type immune complexes formed by capture antibodies immobilized o...

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Bibliographic Details
Main Authors: Kohji Mitsubayashi, Kazuyoshi Yano, Hirokazu Saito, Hiroyuki Kudo, Takahiro Arakawa, Tomoyuki Koshida, Kumiko Miyajima
Format: Article
Language:English
Published: MDPI AG 2013-03-01
Series:Biosensors
Subjects:
E. coli O157:H7
fluorescence
immunoassay
optical fiber
flow cell
Online Access:http://www.mdpi.com/2079-6374/3/1/120

Internet

http://www.mdpi.com/2079-6374/3/1/120

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