In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia
Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023092939 |
| _version_ | 1797429692560572416 |
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| author | Sofia A. Omari Hansen J. Kosasih Thomas Chung Charles E. de Bock |
| author_facet | Sofia A. Omari Hansen J. Kosasih Thomas Chung Charles E. de Bock |
| author_sort | Sofia A. Omari |
| collection | DOAJ |
| description | Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling. These are subsequently used for the viral transduction of the IL-3 cytokine-dependent Ba/F3 cell line or murine hematopoietic stem and progenitor cells (HSPCs) for in vitro and in vivo modelling of cytokine-independent growth or leukemia initiation respectively. |
| first_indexed | 2024-03-09T09:16:49Z |
| format | Article |
| id | doaj.art-6a03e7aa03c54534a97e5458b746fd59 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:16:49Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-6a03e7aa03c54534a97e5458b746fd592023-12-02T07:05:08ZengElsevierHeliyon2405-84402023-11-01911e22085In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemiaSofia A. Omari0Hansen J. Kosasih1Thomas Chung2Charles E. de Bock3Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney 2052, Australia; School of Clinical Medicine, Medicine and Health, UNSW Sydney, Sydney 2052, AustraliaChildren's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney 2052, Australia; School of Clinical Medicine, Medicine and Health, UNSW Sydney, Sydney 2052, AustraliaChildren's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney 2052, Australia; School of Clinical Medicine, Medicine and Health, UNSW Sydney, Sydney 2052, AustraliaChildren's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney 2052, Australia; School of Clinical Medicine, Medicine and Health, UNSW Sydney, Sydney 2052, Australia; Corresponding author. Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney 2052, Australia.Mutations within the IL7-R-JAK-STAT signaling pathway are important drivers of T-cell acute lymphoblastic leukemia (T-ALL). Here we describe the important steps required to generate retroviral particles for the stable expression of mutant JAK3 constructs that induce constitutive JAK/STAT signaling. These are subsequently used for the viral transduction of the IL-3 cytokine-dependent Ba/F3 cell line or murine hematopoietic stem and progenitor cells (HSPCs) for in vitro and in vivo modelling of cytokine-independent growth or leukemia initiation respectively.http://www.sciencedirect.com/science/article/pii/S2405844023092939Bone marrow transplantJAK3STAT5T-cell acute lymphoblastic leukemiaRetroviral transductionTransfection |
| spellingShingle | Sofia A. Omari Hansen J. Kosasih Thomas Chung Charles E. de Bock In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia Heliyon Bone marrow transplant JAK3 STAT5 T-cell acute lymphoblastic leukemia Retroviral transduction Transfection |
| title | In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia |
| title_full | In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia |
| title_fullStr | In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia |
| title_full_unstemmed | In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia |
| title_short | In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in leukemia |
| title_sort | in vitro and in vivo modelling of mutant jak3 stat5 signaling in leukemia |
| topic | Bone marrow transplant JAK3 STAT5 T-cell acute lymphoblastic leukemia Retroviral transduction Transfection |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023092939 |
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