Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells
N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1...
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-11-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/7/4/589/ |
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| author | Remy E. P. Mangindaan Michio Namikoshi Hiroshi Handa Yasuaki Kabe Jong-Soo Lee Satoshi Sakamoto Yuta Sato Taiko Oda |
| author_facet | Remy E. P. Mangindaan Michio Namikoshi Hiroshi Handa Yasuaki Kabe Jong-Soo Lee Satoshi Sakamoto Yuta Sato Taiko Oda |
| author_sort | Remy E. P. Mangindaan |
| collection | DOAJ |
| description | N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis. |
| first_indexed | 2024-04-14T06:28:50Z |
| format | Article |
| id | doaj.art-6a1c06c43de549868069832bf8bfa40c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T06:28:50Z |
| publishDate | 2009-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6a1c06c43de549868069832bf8bfa40c2022-12-22T02:07:42ZengMDPI AGMarine Drugs1660-33972009-11-017458959910.3390/md7040589Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 CellsRemy E. P. MangindaanMichio NamikoshiHiroshi HandaYasuaki KabeJong-Soo LeeSatoshi SakamotoYuta SatoTaiko OdaN,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.http://www.mdpi.com/1660-3397/7/4/589/LPSRAW 264.7 cellsNF-κBTNF-αN,N-didesmethylgrossularine-1natural product |
| spellingShingle | Remy E. P. Mangindaan Michio Namikoshi Hiroshi Handa Yasuaki Kabe Jong-Soo Lee Satoshi Sakamoto Yuta Sato Taiko Oda Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells Marine Drugs LPS RAW 264.7 cells NF-κB TNF-α N,N-didesmethylgrossularine-1 natural product |
| title | Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells |
| title_full | Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells |
| title_fullStr | Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells |
| title_full_unstemmed | Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells |
| title_short | Inhibitory Effect of N,N-Didesmethylgrossularine-1 on Inflammatory Cytokine Production in Lipopolysaccharide-Stimulated RAW 264.7 Cells |
| title_sort | inhibitory effect of n n didesmethylgrossularine 1 on inflammatory cytokine production in lipopolysaccharide stimulated raw 264 7 cells |
| topic | LPS RAW 264.7 cells NF-κB TNF-α N,N-didesmethylgrossularine-1 natural product |
| url | http://www.mdpi.com/1660-3397/7/4/589/ |
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