Cost-effective sequencing of full-length cDNA clones powered by a de novo-reference hybrid assembly.

BACKGROUND: Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the biological functions of coded proteins. However, previous approaches for sequencing cDNA clones were ex...

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Bibliographic Details
Main Authors: Reginaldo M Kuroshu, Junichi Watanabe, Sumio Sugano, Shinichi Morishita, Yutaka Suzuki, Masahiro Kasahara
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2866332?pdf=render