A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST

A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST

NanoFAST is the smallest fluorogen-activating protein, consisting of only 98 amino acids, used as a genetically encoded fluorescent tag. Previously, only a single fluorogen with an orange color was revealed for this protein. In the present paper, using rational mutagenesis and in vitro screening of...

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Bibliographic Details
Main Authors: Nadezhda S. Baleeva, Yulia A. Bogdanova, Marina V. Goncharuk, Anatolii I. Sokolov, Ivan N. Myasnyanko, Vadim S. Kublitski, Alexander Yu. Smirnov, Aidar R. Gilvanov, Sergey A. Goncharuk, Konstantin S. Mineev, Mikhail S. Baranov
Format: Article
Language:English
Published: MDPI AG 2024-03-01
Series:International Journal of Molecular Sciences
Subjects:
fluorogen-activating proteins
FAST
fluorogen
fluorescence
nanoFAST
Online Access:https://www.mdpi.com/1422-0067/25/5/3054

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https://www.mdpi.com/1422-0067/25/5/3054

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