An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the prep...

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Bibliographic Details
Main Authors: Peter A. Lohse, Guannan Kuang, Markus Kurz
Format: Article
Language:English
Published: MDPI AG 2000-12-01
Series:Molecules
Subjects:
Nucleic acid-encoded libraries
mRNA-protein fusion
PROfusionTM
in vitro selection
puromycin
psoralen
Online Access:http://www.mdpi.com/1420-3049/5/12/1259/

Internet

http://www.mdpi.com/1420-3049/5/12/1259/

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