An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the prep...

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Bibliographic Details
Main Authors: Peter A. Lohse, Guannan Kuang, Markus Kurz
Format: Article
Language:English
Published: MDPI AG 2000-12-01
Series:Molecules
Subjects:
Nucleic acid-encoded libraries
mRNA-protein fusion
PROfusionTM
in vitro selection
puromycin
psoralen
Online Access:http://www.mdpi.com/1420-3049/5/12/1259/
Description
Summary:We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.
ISSN:1420-3049

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