An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols
We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the prep...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2000-12-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/5/12/1259/ |
| _version_ | 1811275330858516480 |
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| author | Peter A. Lohse Guannan Kuang Markus Kurz |
| author_facet | Peter A. Lohse Guannan Kuang Markus Kurz |
| author_sort | Peter A. Lohse |
| collection | DOAJ |
| description | We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection. |
| first_indexed | 2024-04-12T23:35:52Z |
| format | Article |
| id | doaj.art-6a2b3bc13a1f4eb68f350c0473fc4d0d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T23:35:52Z |
| publishDate | 2000-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6a2b3bc13a1f4eb68f350c0473fc4d0d2022-12-22T03:12:08ZengMDPI AGMolecules1420-30492000-12-015121259126410.3390/51201259An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution ProtocolsPeter A. LohseGuannan KuangMarkus KurzWe describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.http://www.mdpi.com/1420-3049/5/12/1259/Nucleic acid-encoded librariesmRNA-protein fusionPROfusionTMin vitro selectionpuromycinpsoralen |
| spellingShingle | Peter A. Lohse Guannan Kuang Markus Kurz An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols Molecules Nucleic acid-encoded libraries mRNA-protein fusion PROfusionTM in vitro selection puromycin psoralen |
| title | An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols |
| title_full | An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols |
| title_fullStr | An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols |
| title_full_unstemmed | An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols |
| title_short | An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols |
| title_sort | efficient synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries for in vitro evolution protocols |
| topic | Nucleic acid-encoded libraries mRNA-protein fusion PROfusionTM in vitro selection puromycin psoralen |
| url | http://www.mdpi.com/1420-3049/5/12/1259/ |
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