Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

Summary: Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We...

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Bibliographic Details
Main Authors: J. Eduardo Pérez-Mojica, Lennart Enders, Kin H. Lau, Adelheid Lempradl
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Developmental Biology
RNAseq
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005026

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http://www.sciencedirect.com/science/article/pii/S2666166723005026

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