Direct transfer of genetic material between two Escherichia coli K12 strains by electroporation

A direct transfer of plasmid pBP517 from C600 to J53 Escherichia coli K12 strains by electroporation, either by the standard method, or in the presence of 20 μg/mL of DNAse was carried out. In a standard experiment, donor and recipient bacteria were mixed and subjected to the electroporation procedu...

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Bibliographic Details
Main Authors: Anna Marchese, Eugenio A. Debbia
Format: Article
Language:English
Published: MDPI AG 2017-11-01
Series:Microbiology Research
Subjects:
Online Access:http://www.pagepress.org/journals/index.php/mr/article/view/7311