Loop optimization of Trichoderma reesei endoglucanases for balancing the activity–stability trade‐off through cross‐strategy between machine learning and the B‐factor analysis

Loop optimization of Trichoderma reesei endoglucanases for balancing the activity–stability trade‐off through cross‐strategy between machine learning and the B‐factor analysis

Abstract Trichoderma reesei endoglucanases (EGs) have limited industrial applications due to its low thermostability and activity. Here, we aimed to improve the thermostability of EGs from T. reesei without reducing its activity counteracting the activity–stability trade‐off. A cross‐strategy combin...

Full description

Bibliographic Details
Main Authors: Le Gao, Qi Guo, Ruinan Xu, Haofan Dong, Chichun Zhou, Zhuohang Yu, Zhaokun Zhang, Lixian Wang, Xiaoyi Chen, Xin Wu
Format: Article
Language:English
Published: Wiley 2023-02-01
Series:GCB Bioenergy
Subjects:
activity–stability trade‐off
B‐factor
endoglucanase
loop optimization
machine learning approaches
thermostability
Online Access:https://doi.org/10.1111/gcbb.13011

Internet

https://doi.org/10.1111/gcbb.13011

Similar Items