Loop optimization of Trichoderma reesei endoglucanases for balancing the activity–stability trade‐off through cross‐strategy between machine learning and the B‐factor analysis
Abstract Trichoderma reesei endoglucanases (EGs) have limited industrial applications due to its low thermostability and activity. Here, we aimed to improve the thermostability of EGs from T. reesei without reducing its activity counteracting the activity–stability trade‐off. A cross‐strategy combin...
Main Authors: | Le Gao, Qi Guo, Ruinan Xu, Haofan Dong, Chichun Zhou, Zhuohang Yu, Zhaokun Zhang, Lixian Wang, Xiaoyi Chen, Xin Wu |
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Format: | Article |
Language: | English |
Published: |
Wiley
2023-02-01
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Series: | GCB Bioenergy |
Subjects: | |
Online Access: | https://doi.org/10.1111/gcbb.13011 |
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