Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 se...
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MDPI AG
2011-01-01
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author | Jun Zhang Mei-Lin Liu Ling-Yun Zhai Hong-Ru Jiang Xiao-Li Xu Jie Wang Wei Li |
author_facet | Jun Zhang Mei-Lin Liu Ling-Yun Zhai Hong-Ru Jiang Xiao-Li Xu Jie Wang Wei Li |
author_sort | Jun Zhang |
collection | DOAJ |
description | The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. The inhibitory rate on tumor growth in the combination group was 35.54% which is significant statistically compared with that of the control group (P < 0.05). In the combination group, the index of cell proliferation and apoptosis were 12.40% and 51.03% respectively, which are significant statistically compared with those of the control group (22.56%, 19.07%, all P < 0.05). These studies indicate that synergism between two COX inhibitors and inhibitor combination treatment has particular potential for chemoprevention of ovarian cancer growth. |
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spelling | doaj.art-6a41172923084fceb69c66fd362c38162022-12-22T03:05:07ZengMDPI AGInternational Journal of Molecular Sciences1422-00672011-01-0112166868110.3390/ijms12010668Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in VivoJun ZhangMei-Lin LiuLing-Yun ZhaiHong-Ru JiangXiao-Li XuJie WangWei LiThe present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. The inhibitory rate on tumor growth in the combination group was 35.54% which is significant statistically compared with that of the control group (P < 0.05). In the combination group, the index of cell proliferation and apoptosis were 12.40% and 51.03% respectively, which are significant statistically compared with those of the control group (22.56%, 19.07%, all P < 0.05). These studies indicate that synergism between two COX inhibitors and inhibitor combination treatment has particular potential for chemoprevention of ovarian cancer growth.http://www.mdpi.com/1422-0067/12/1/668/ovarian carcinomamicecyclooxygenase selective inhibitorcell proliferationapoptosis |
spellingShingle | Jun Zhang Mei-Lin Liu Ling-Yun Zhai Hong-Ru Jiang Xiao-Li Xu Jie Wang Wei Li Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo International Journal of Molecular Sciences ovarian carcinoma mice cyclooxygenase selective inhibitor cell proliferation apoptosis |
title | Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo |
title_full | Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo |
title_fullStr | Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo |
title_full_unstemmed | Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo |
title_short | Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo |
title_sort | combined effects of cyclooxygenase 1 and cyclooxygenase 2 selective inhibitors on ovarian carcinoma in vivo |
topic | ovarian carcinoma mice cyclooxygenase selective inhibitor cell proliferation apoptosis |
url | http://www.mdpi.com/1422-0067/12/1/668/ |
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