Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers

Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers

Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these cultures in less time. In the present study, we e...

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Bibliographic Details
Main Authors: Jesús Chato-Astrain, David Sánchez-Porras, Óscar Darío García-García, Claudia Vairo, María Villar-Vidal, Silvia Villullas, Indalecio Sánchez-Montesinos, Fernando Campos, Ingrid Garzón, Miguel Alaminos
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:Biomedicines
Subjects:
EGF
nanoparticles
NLC
keratinocytes
tissue engineering
cell culture
Online Access:https://www.mdpi.com/2227-9059/9/11/1634

Internet

https://www.mdpi.com/2227-9059/9/11/1634

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