Orthogonal translation enables heterologous ribosome engineering in E. coli
Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-01-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-20759-z |