Orthogonal translation enables heterologous ribosome engineering in E. coli

Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize...

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Bibliographic Details
Main Authors: Natalie S. Kolber, Ranan Fattal, Sinisa Bratulic, Gavriela D. Carver, Ahmed H. Badran
Format: Article
Language:English
Published: Nature Portfolio 2021-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20759-z