Orthogonal translation enables heterologous ribosome engineering in E. coli
Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20759-z |
| _version_ | 1818749463168024576 |
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| author | Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran |
| author_facet | Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran |
| author_sort | Natalie S. Kolber |
| collection | DOAJ |
| description | Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize their function. |
| first_indexed | 2024-12-18T04:04:10Z |
| format | Article |
| id | doaj.art-6aa4a18ef0ce446fb94992971a6c0623 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T04:04:10Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6aa4a18ef0ce446fb94992971a6c06232022-12-21T21:21:38ZengNature PortfolioNature Communications2041-17232021-01-0112111210.1038/s41467-020-20759-zOrthogonal translation enables heterologous ribosome engineering in E. coliNatalie S. Kolber0Ranan Fattal1Sinisa Bratulic2Gavriela D. Carver3Ahmed H. Badran4Broad Institute of MIT and HarvardBroad Institute of MIT and HarvardBroad Institute of MIT and HarvardBroad Institute of MIT and HarvardBroad Institute of MIT and HarvardSynthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize their function.https://doi.org/10.1038/s41467-020-20759-z |
| spellingShingle | Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran Orthogonal translation enables heterologous ribosome engineering in E. coli Nature Communications |
| title | Orthogonal translation enables heterologous ribosome engineering in E. coli |
| title_full | Orthogonal translation enables heterologous ribosome engineering in E. coli |
| title_fullStr | Orthogonal translation enables heterologous ribosome engineering in E. coli |
| title_full_unstemmed | Orthogonal translation enables heterologous ribosome engineering in E. coli |
| title_short | Orthogonal translation enables heterologous ribosome engineering in E. coli |
| title_sort | orthogonal translation enables heterologous ribosome engineering in e coli |
| url | https://doi.org/10.1038/s41467-020-20759-z |
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