Protocol for aerosolization challenge of mice with Bordetella pertussis

Summary: Bordetella pertussis causes whooping cough and is transmitted via respiratory droplets. Here, we present a protocol to challenge mice with Bordetella pertussis. We describe bacteria preparation and long-term storage, followed by manufacturing a challenge dose for use in a commercial exposur...

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Bibliographic Details
Main Authors: Graham Bitzer, Kelly Weaver, Mariette Barbier, F. Heath Damron
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008590