A tool for CRISPR-Cas9 sgRNA evaluation based on computational models of gene expression
Abstract Background CRISPR is widely used to silence genes by inducing mutations expected to nullify their expression. While numerous computational tools have been developed to design single-guide RNAs (sgRNAs) with high cutting efficiency and minimal off-target effects, only a few tools focus speci...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
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| Series: | Genome Medicine |
| Online Access: | https://doi.org/10.1186/s13073-024-01420-6 |