Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene
To investigate the effect of <i>n</i>-butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo ‘Hami’), the fruits were soaked in a 1.0% solution of <i>n</i>-butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly,...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/9/1904 |
| _version_ | 1797602622278991872 |
|---|---|
| author | Shuai Huang Ying Bi Hui Li Caihong Liu Xue Wang Xinyu Wang Yaxin Lei Qi Zhang Jing Wang |
| author_facet | Shuai Huang Ying Bi Hui Li Caihong Liu Xue Wang Xinyu Wang Yaxin Lei Qi Zhang Jing Wang |
| author_sort | Shuai Huang |
| collection | DOAJ |
| description | To investigate the effect of <i>n</i>-butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo ‘Hami’), the fruits were soaked in a 1.0% solution of <i>n</i>-butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly, and the indices related to permeability and membrane lipid metabolism of pericarp cells were measured. The results showed that treatment with <i>n</i>-butanol inhibited the increase in chilling injury index, membrane permeability, and malondialdehyde content of Hami melon fruits, promoted an increase in the contents of phosphatidyl alcohol and unsaturated fatty acids, such as linoleic acid, linolenic acid, oleic acid (except 14 d), and erucic acid (28–42 d), and decreased the content of saturated fatty acids, stearic acid (0–28 d), phosphatidic acid (except for 21 d), and the key enzymes of membrane lipid metabolism compared with the control. The activities of phospholipase D (PLD) and lipoxygenase (LOX) and the downregulation of the levels of expression <i>CmPLD-β</i> and <i>CmLOX</i> (42 d only) genes reduced the chilling injury index of Hami melon and alleviated the further expansion of chilling injury symptoms in the fruits. We also cloned the key gene of membrane lipid metabolism <i>CmPLD-β</i>, which was obtained by pre-transcriptome screening of the pericarp. We found that <i>CmPLD-β</i> of Hami melon had the closest affinity with cucumber (CsXP5), indicating that the <i>CmPLD-β</i> gene of Hami melon was functionally similar to that of cucumber. In addition, a two-fold alignment analysis of <i>CmPLD-β</i> and CmXP5 base sequences indicated that the base sequences of the two promoter regions differed from each other. |
| first_indexed | 2024-03-11T04:19:13Z |
| format | Article |
| id | doaj.art-6af2dbed367046c6b028f7ba4d493f44 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T04:19:13Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6af2dbed367046c6b028f7ba4d493f442023-11-17T22:56:26ZengMDPI AGFoods2304-81582023-05-01129190410.3390/foods12091904Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β GeneShuai Huang0Ying Bi1Hui Li2Caihong Liu3Xue Wang4Xinyu Wang5Yaxin Lei6Qi Zhang7Jing Wang8College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaTo investigate the effect of <i>n</i>-butanol on postharvest membrane lipid metabolism of Hami melon (Cucumis melo ‘Hami’), the fruits were soaked in a 1.0% solution of <i>n</i>-butanol for 30 min with water as the control. Symptoms of chilling injury were observed regularly, and the indices related to permeability and membrane lipid metabolism of pericarp cells were measured. The results showed that treatment with <i>n</i>-butanol inhibited the increase in chilling injury index, membrane permeability, and malondialdehyde content of Hami melon fruits, promoted an increase in the contents of phosphatidyl alcohol and unsaturated fatty acids, such as linoleic acid, linolenic acid, oleic acid (except 14 d), and erucic acid (28–42 d), and decreased the content of saturated fatty acids, stearic acid (0–28 d), phosphatidic acid (except for 21 d), and the key enzymes of membrane lipid metabolism compared with the control. The activities of phospholipase D (PLD) and lipoxygenase (LOX) and the downregulation of the levels of expression <i>CmPLD-β</i> and <i>CmLOX</i> (42 d only) genes reduced the chilling injury index of Hami melon and alleviated the further expansion of chilling injury symptoms in the fruits. We also cloned the key gene of membrane lipid metabolism <i>CmPLD-β</i>, which was obtained by pre-transcriptome screening of the pericarp. We found that <i>CmPLD-β</i> of Hami melon had the closest affinity with cucumber (CsXP5), indicating that the <i>CmPLD-β</i> gene of Hami melon was functionally similar to that of cucumber. In addition, a two-fold alignment analysis of <i>CmPLD-β</i> and CmXP5 base sequences indicated that the base sequences of the two promoter regions differed from each other.https://www.mdpi.com/2304-8158/12/9/1904<i>n</i>-butanolHami melonchilling injurymembrane lipid metabolismgene cloning |
| spellingShingle | Shuai Huang Ying Bi Hui Li Caihong Liu Xue Wang Xinyu Wang Yaxin Lei Qi Zhang Jing Wang Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene Foods <i>n</i>-butanol Hami melon chilling injury membrane lipid metabolism gene cloning |
| title | Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene |
| title_full | Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene |
| title_fullStr | Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene |
| title_full_unstemmed | Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene |
| title_short | Reduction of Membrane Lipid Metabolism in Postharvest Hami Melon Fruits by <i>n</i>-Butanol to Mitigate Chilling Injury and the Cloning of Phospholipase D-β Gene |
| title_sort | reduction of membrane lipid metabolism in postharvest hami melon fruits by i n i butanol to mitigate chilling injury and the cloning of phospholipase d β gene |
| topic | <i>n</i>-butanol Hami melon chilling injury membrane lipid metabolism gene cloning |
| url | https://www.mdpi.com/2304-8158/12/9/1904 |
| work_keys_str_mv | AT shuaihuang reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT yingbi reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT huili reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT caihongliu reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT xuewang reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT xinyuwang reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT yaxinlei reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT qizhang reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene AT jingwang reductionofmembranelipidmetabolisminpostharvesthamimelonfruitsbyinibutanoltomitigatechillinginjuryandthecloningofphospholipasedbgene |