Selection and Evaluation of mRNA and miRNA Reference Genes for Expression Studies (qPCR) in Archived Formalin-Fixed and Paraffin-Embedded (FFPE) Colon Samples of DSS-Induced Colitis Mouse Model

Selection and Evaluation of mRNA and miRNA Reference Genes for Expression Studies (qPCR) in Archived Formalin-Fixed and Paraffin-Embedded (FFPE) Colon Samples of DSS-Induced Colitis Mouse Model

The choice of appropriate reference genes is essential for correctly interpreting qPCR data and results. However, the majority of animal studies use a single reference gene without any prior evaluation. Therefore, many qPCR results from rodent studies can be misleading, affecting not only reproducib...

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Bibliographic Details
Main Authors: Ana Unkovič, Emanuela Boštjančič, Aleš Belič, Martina Perše
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Biology
Subjects:
reference gene
archived FFPE
DSS model
mouse
mRNA
miRNA
Online Access:https://www.mdpi.com/2079-7737/12/2/190

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https://www.mdpi.com/2079-7737/12/2/190

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