Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors

Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors

DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing fluorescence super-resolution technique, which allows for reaching spatial resolutions below 10 nm. It also enables the imaging of multiple targets in the same sample. However, using DNA-PAINT to observe cellu...

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Bibliographic Details
Main Authors: Shama Sograte-Idrissi, Nazar Oleksiievets, Sebastian Isbaner, Mariana Eggert-Martinez, Jörg Enderlein, Roman Tsukanov, Felipe Opazo
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Cells
Subjects:
nanobodies
super-resolution microscopy
multi-color imaging
fluorescent proteins
microfluidics
DNA-PAINT
molecular localization
single domain antibodies (sdAb)
multiplexing
linkage error
Online Access:http://www.mdpi.com/2073-4409/8/1/48

Internet

http://www.mdpi.com/2073-4409/8/1/48

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