Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors

Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors

DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing fluorescence super-resolution technique, which allows for reaching spatial resolutions below 10 nm. It also enables the imaging of multiple targets in the same sample. However, using DNA-PAINT to observe cellu...

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Bibliographic Details
Main Authors:	Shama Sograte-Idrissi, Nazar Oleksiievets, Sebastian Isbaner, Mariana Eggert-Martinez, Jörg Enderlein, Roman Tsukanov, Felipe Opazo
Format:	Article
Language:	English
Published:	MDPI AG 2019-01-01
Series:	Cells
Subjects:	nanobodies super-resolution microscopy multi-color imaging fluorescent proteins microfluidics DNA-PAINT molecular localization single domain antibodies (sdAb) multiplexing linkage error
Online Access:	http://www.mdpi.com/2073-4409/8/1/48

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