Enhanced transgene expression from single-stranded AAV vectors in human cells in vitro and in murine hepatocytes in vivo

Enhanced transgene expression from single-stranded AAV vectors in human cells in vitro and in murine hepatocytes in vivo

We identified that distal 10 nucleotides in the D-sequence in AAV2 inverted terminal repeat (ITR) share partial sequence homology to 1/2 binding site of glucocorticoid receptor-binding element (GRE). Here, we describe that (1) purified GR binds to AAV2 D-sequence, and the D-sequence competes with GR...

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Bibliographic Details
Main Authors: Yuan Lu, Chen Ling, Jakob Shoti, Hua Yang, Aneesha Nath, Geoffrey D. Keeler, Keyun Qing, Arun Srivastava
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: Delivery Strategies
AAV vectors
gene transfer
glucocorticoid receptor-binding element
transgene expression
gene therapy
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253124000830

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http://www.sciencedirect.com/science/article/pii/S2162253124000830

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