Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion
Summary: To thrive in nature, bacteria have to reproduce efficiently under favorable conditions and persist during stress. The global strategy that integrates the growth control and stress response remains to be explored. Here, we find that a moderate induction of (p)ppGpp reduces growth rate but si...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224000397 |
| _version_ | 1827378741875048448 |
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| author | Manlu Zhu Haoyan Mu Xiongfeng Dai |
| author_facet | Manlu Zhu Haoyan Mu Xiongfeng Dai |
| author_sort | Manlu Zhu |
| collection | DOAJ |
| description | Summary: To thrive in nature, bacteria have to reproduce efficiently under favorable conditions and persist during stress. The global strategy that integrates the growth control and stress response remains to be explored. Here, we find that a moderate induction of (p)ppGpp reduces growth rate but significantly enhances the stress tolerance of E. coli, resulting from a global resource re-allocation from ribosome synthesis to the synthesis of stress-responsive proteins. Strikingly, the activation of stress response by (p)ppGpp is still largely retained in the absence of RpoS. In addition, (p)ppGpp induction could activate the catabolism of alanine and arginine, facilitating the adaption of bacteria to nutrient downshift. Our work demonstrates that the activation of stress response by (p)ppGpp could occur in an RpoS-independent manner and (p)ppGpp enables bacteria to integrate the control of growth and stress response in a seesaw fashion, thus acting as an important global regulator of the bacterial fitness landscape. |
| first_indexed | 2024-03-08T13:02:15Z |
| format | Article |
| id | doaj.art-6b6966e2f2774ed7abf4176ab192f524 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T13:02:15Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6b6966e2f2774ed7abf4176ab192f5242024-01-19T05:01:53ZengElsevieriScience2589-00422024-02-01272108818Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashionManlu Zhu0Haoyan Mu1Xiongfeng Dai2Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences & National Key Laboratory of Green Pesticides, Central China Normal University, Wuhan, China; Corresponding authorHubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences & National Key Laboratory of Green Pesticides, Central China Normal University, Wuhan, ChinaHubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences & National Key Laboratory of Green Pesticides, Central China Normal University, Wuhan, China; Corresponding authorSummary: To thrive in nature, bacteria have to reproduce efficiently under favorable conditions and persist during stress. The global strategy that integrates the growth control and stress response remains to be explored. Here, we find that a moderate induction of (p)ppGpp reduces growth rate but significantly enhances the stress tolerance of E. coli, resulting from a global resource re-allocation from ribosome synthesis to the synthesis of stress-responsive proteins. Strikingly, the activation of stress response by (p)ppGpp is still largely retained in the absence of RpoS. In addition, (p)ppGpp induction could activate the catabolism of alanine and arginine, facilitating the adaption of bacteria to nutrient downshift. Our work demonstrates that the activation of stress response by (p)ppGpp could occur in an RpoS-independent manner and (p)ppGpp enables bacteria to integrate the control of growth and stress response in a seesaw fashion, thus acting as an important global regulator of the bacterial fitness landscape.http://www.sciencedirect.com/science/article/pii/S2589004224000397Molecular biologyBacteriologyProteomics |
| spellingShingle | Manlu Zhu Haoyan Mu Xiongfeng Dai Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion iScience Molecular biology Bacteriology Proteomics |
| title | Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion |
| title_full | Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion |
| title_fullStr | Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion |
| title_full_unstemmed | Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion |
| title_short | Integrated control of bacterial growth and stress response by (p)ppGpp in Escherichia coli: A seesaw fashion |
| title_sort | integrated control of bacterial growth and stress response by p ppgpp in escherichia coli a seesaw fashion |
| topic | Molecular biology Bacteriology Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224000397 |
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