Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11
Mucor circinelloides serves as a model organism to investigate the lipid metabolism in oleaginous microorganisms. It is considered as an important producer of γ-linolenic acid (GLA) that has vital medicinal benefits. In this study, we used WJ11, a high lipid-producing strain of M. circinelloides (36...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.919364/full |
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| author | Abu Bakr Ahmad Fazili Aabid Manzoor Shah Mohammed Fahad Albeshr Tahira Naz Mohammad Abass Dar Wu Yang Victoriano Garre Khalid Majid Fazili Eijaz Ahmed Bhat Yuanda Song |
| author_facet | Abu Bakr Ahmad Fazili Aabid Manzoor Shah Mohammed Fahad Albeshr Tahira Naz Mohammad Abass Dar Wu Yang Victoriano Garre Khalid Majid Fazili Eijaz Ahmed Bhat Yuanda Song |
| author_sort | Abu Bakr Ahmad Fazili |
| collection | DOAJ |
| description | Mucor circinelloides serves as a model organism to investigate the lipid metabolism in oleaginous microorganisms. It is considered as an important producer of γ-linolenic acid (GLA) that has vital medicinal benefits. In this study, we used WJ11, a high lipid-producing strain of M. circinelloides (36% w/w lipid, cell dry weight, CDW), to examine the role in lipid accumulation of two mitochondrial malic enzyme (ME) genes malC and malD. The homologous overexpression of both malC and malD genes enhanced the total lipid content of WJ11 by 41.16 and 32.34%, respectively. In parallel, the total content of GLA was enhanced by 16.73 and 46.76% in malC and malD overexpressing strains, respectively, because of the elevation of total lipid content. The fact that GLA content was enhanced more in the strain with lower lipid content increase and vice versa, indicated that engineering of mitochondrial MEs altered the fatty acid profile. Our results reveal that mitochondrial ME plays an important role in lipid metabolism and suggest that future approaches may involve simultaneous overexpression of distinct ME genes to boost lipid accumulation even further. |
| first_indexed | 2024-12-12T08:16:12Z |
| format | Article |
| id | doaj.art-6b9dd0260b3f4cac872e90731ff008bf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T08:16:12Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6b9dd0260b3f4cac872e90731ff008bf2022-12-22T00:31:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.919364919364Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11Abu Bakr Ahmad Fazili0Aabid Manzoor Shah1Mohammed Fahad Albeshr2Tahira Naz3Mohammad Abass Dar4Wu Yang5Victoriano Garre6Khalid Majid Fazili7Eijaz Ahmed Bhat8Yuanda Song9Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaDepartment of Biotechnology, University of Kashmir, Srinagar, IndiaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaDepartamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, Murcia, SpainDepartment of Biotechnology, University of Kashmir, Srinagar, IndiaCentre de Biologie Structurale (CBS), Univ. Montpellier, CNRS, INSERM, Montpellier, FranceColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, ChinaMucor circinelloides serves as a model organism to investigate the lipid metabolism in oleaginous microorganisms. It is considered as an important producer of γ-linolenic acid (GLA) that has vital medicinal benefits. In this study, we used WJ11, a high lipid-producing strain of M. circinelloides (36% w/w lipid, cell dry weight, CDW), to examine the role in lipid accumulation of two mitochondrial malic enzyme (ME) genes malC and malD. The homologous overexpression of both malC and malD genes enhanced the total lipid content of WJ11 by 41.16 and 32.34%, respectively. In parallel, the total content of GLA was enhanced by 16.73 and 46.76% in malC and malD overexpressing strains, respectively, because of the elevation of total lipid content. The fact that GLA content was enhanced more in the strain with lower lipid content increase and vice versa, indicated that engineering of mitochondrial MEs altered the fatty acid profile. Our results reveal that mitochondrial ME plays an important role in lipid metabolism and suggest that future approaches may involve simultaneous overexpression of distinct ME genes to boost lipid accumulation even further.https://www.frontiersin.org/articles/10.3389/fmicb.2022.919364/fulllipid accumulationMucor circinelloidesoleaginous fungimalic enzymeγ-linolenic acidgenetic engineering |
| spellingShingle | Abu Bakr Ahmad Fazili Aabid Manzoor Shah Mohammed Fahad Albeshr Tahira Naz Mohammad Abass Dar Wu Yang Victoriano Garre Khalid Majid Fazili Eijaz Ahmed Bhat Yuanda Song Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 Frontiers in Microbiology lipid accumulation Mucor circinelloides oleaginous fungi malic enzyme γ-linolenic acid genetic engineering |
| title | Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 |
| title_full | Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 |
| title_fullStr | Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 |
| title_full_unstemmed | Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 |
| title_short | Overexpression of the Mitochondrial Malic Enzyme Genes (malC and malD) Improved the Lipid Accumulation in Mucor circinelloides WJ11 |
| title_sort | overexpression of the mitochondrial malic enzyme genes malc and mald improved the lipid accumulation in mucor circinelloides wj11 |
| topic | lipid accumulation Mucor circinelloides oleaginous fungi malic enzyme γ-linolenic acid genetic engineering |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.919364/full |
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