Time-Gated Luminescent In Situ Hybridization (LISH): Highly Sensitive Detection of Pathogenic <i>Staphylococcus aureus</i>

Time-Gated Luminescent In Situ Hybridization (LISH): Highly Sensitive Detection of Pathogenic <i>Staphylococcus aureus</i>

We describe simple direct conjugation of a single TEGylated Europium chelate to DNA that binds to intracellular rRNA and is then detected using a homogeneous luminescent in situ hybridisation (LISH) technique. As a proof-of-principle, <i>Staphylococcus aureus (S. aureus)</i> was selected...

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Bibliographic Details
Main Authors: Nima Sayyadi, Russell E. Connally, Thomas S. Lawson, Jingli Yuan, Nicolle H. Packer, James A. Piper
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Molecules
Subjects:
homogeneous luminescent in situ hybridisation
TEGylated Europium chelate
time-gated luminescent microscopy
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) detection
Online Access:https://www.mdpi.com/1420-3049/24/11/2083

Internet

https://www.mdpi.com/1420-3049/24/11/2083

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