Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples
Efficiency of three different sample preparation methods for GC/MS determinationof bifenthrin residues in wheat (Triticum spelta) samples fermented by Lactobacillusplantarum was tested. The first method was based on a methanol:acetone=1:1 extractionfolowed by a purification on columns containing mix...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Pesticides and Environmental Protection
2012-01-01
|
| Series: | Pesticidi i Fitomedicina |
| Subjects: | |
| Online Access: | http://www.pesting.org.rs/download.php/documents/27-2/27-2%20167-174.pdf |
| _version_ | 1828173893206016000 |
|---|---|
| author | Tijana Đorđević Rada Đurović Jelena Gajić-Umiljendić |
| author_facet | Tijana Đorđević Rada Đurović Jelena Gajić-Umiljendić |
| author_sort | Tijana Đorđević |
| collection | DOAJ |
| description | Efficiency of three different sample preparation methods for GC/MS determinationof bifenthrin residues in wheat (Triticum spelta) samples fermented by Lactobacillusplantarum was tested. The first method was based on a methanol:acetone=1:1 extractionfolowed by a purification on columns containing mixture of aluminium oxide and activatedcharcoal slurry-packed and eluted with dichlormethane, the second was based onmethanol:acetone=1:1 extraction folowed by the purification on florisil column and elutionby ethil acetate:acetone=4:1, while the third tested method was based on a combinationof the first two mentioned methods, thus methanol:acetone=1:1 extraction and clean-upthrought columns filled with a mixture of aluminum oxide and activated charcoal slurrypackedand eluted with ethil acetate:acetone=4:1. The second method was the most effectivefor obtaining satisfactory recoveries for bifenthrin in a range of 79-83% for four fortificationlevels, with good reproducibility i.e. RSD% in a range of 2.2-7.4%. The chosen methodwas further optimized by assessing the optimum volume of elution solvent used duringthe clean-up procedures. The highest recovery of 82.1% was obtained after elution with25 ml of solvent. Overall, two-step extraction with 25 ml of methanol:acetone=1:1 solventmix for 30 min, followed by clean-up procedure through a glass column with florisil coupledwith elution with 25 ml of ethyl acetate: acetone=4:1, allows simple, efficient and reliableGC/MS detection of bifenthrin residues from wheat grain fermented by L. plantarum. |
| first_indexed | 2024-04-12T04:02:46Z |
| format | Article |
| id | doaj.art-6beab61e6cf5467b9990dd1d40deb13c |
| institution | Directory Open Access Journal |
| issn | 1820-3949 |
| language | English |
| last_indexed | 2024-04-12T04:02:46Z |
| publishDate | 2012-01-01 |
| publisher | Institute of Pesticides and Environmental Protection |
| record_format | Article |
| series | Pesticidi i Fitomedicina |
| spelling | doaj.art-6beab61e6cf5467b9990dd1d40deb13c2022-12-22T03:48:40ZengInstitute of Pesticides and Environmental ProtectionPesticidi i Fitomedicina1820-39492012-01-01272167174Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat SamplesTijana ĐorđevićRada ĐurovićJelena Gajić-UmiljendićEfficiency of three different sample preparation methods for GC/MS determinationof bifenthrin residues in wheat (Triticum spelta) samples fermented by Lactobacillusplantarum was tested. The first method was based on a methanol:acetone=1:1 extractionfolowed by a purification on columns containing mixture of aluminium oxide and activatedcharcoal slurry-packed and eluted with dichlormethane, the second was based onmethanol:acetone=1:1 extraction folowed by the purification on florisil column and elutionby ethil acetate:acetone=4:1, while the third tested method was based on a combinationof the first two mentioned methods, thus methanol:acetone=1:1 extraction and clean-upthrought columns filled with a mixture of aluminum oxide and activated charcoal slurrypackedand eluted with ethil acetate:acetone=4:1. The second method was the most effectivefor obtaining satisfactory recoveries for bifenthrin in a range of 79-83% for four fortificationlevels, with good reproducibility i.e. RSD% in a range of 2.2-7.4%. The chosen methodwas further optimized by assessing the optimum volume of elution solvent used duringthe clean-up procedures. The highest recovery of 82.1% was obtained after elution with25 ml of solvent. Overall, two-step extraction with 25 ml of methanol:acetone=1:1 solventmix for 30 min, followed by clean-up procedure through a glass column with florisil coupledwith elution with 25 ml of ethyl acetate: acetone=4:1, allows simple, efficient and reliableGC/MS detection of bifenthrin residues from wheat grain fermented by L. plantarum.http://www.pesting.org.rs/download.php/documents/27-2/27-2%20167-174.pdfWheatFermentationPesticidesResidues |
| spellingShingle | Tijana Đorđević Rada Đurović Jelena Gajić-Umiljendić Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples Pesticidi i Fitomedicina Wheat Fermentation Pesticides Residues |
| title | Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples |
| title_full | Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples |
| title_fullStr | Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples |
| title_full_unstemmed | Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples |
| title_short | Comparison of Methods for Bifenthrin Residues Determination in Fermented Wheat Samples |
| title_sort | comparison of methods for bifenthrin residues determination in fermented wheat samples |
| topic | Wheat Fermentation Pesticides Residues |
| url | http://www.pesting.org.rs/download.php/documents/27-2/27-2%20167-174.pdf |
| work_keys_str_mv | AT tijanađorđevic comparisonofmethodsforbifenthrinresiduesdeterminationinfermentedwheatsamples AT radađurovic comparisonofmethodsforbifenthrinresiduesdeterminationinfermentedwheatsamples AT jelenagajicumiljendic comparisonofmethodsforbifenthrinresiduesdeterminationinfermentedwheatsamples |