Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

Summary: The optimization of the CRISPR-Cas9 system for enhancing editing efficiency holds significant value in scientific research. In this study, we optimized single guide RNA and Cas9 promoters of the CRISPR-Cas9 vector and established an efficient protoplast isolation and transient transformatio...

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Bibliographic Details
Main Authors: Xueqi Li, Fanqi Bu, Lishan Wang, Cholmin Kim, Wanjie Xue, Man Zhang, Saneyuki Kawabata, Qingzhu Zhang, Yuhua Li, Yang Zhang
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:iScience
Subjects:
Molecular biology
Plant Biology
Plant biotechnology
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004224002748

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http://www.sciencedirect.com/science/article/pii/S2589004224002748

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