Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis
The global consumption of vegan foods is experiencing an expressive upward trend, underscoring the critical need for quality control measures based on nutritional and functional considerations. This study aimed to evaluate the functional quality of caviar and salmon analog food inks based on pulses...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/13/7/751 |
| _version_ | 1797590033644912640 |
|---|---|
| author | Gabriella Magarelli Cínthia Caetano Bonatto Gabriela Mendes da Rocha Vaz Victoria Baggi Mendonça Lauria Luciano Paulino Silva |
| author_facet | Gabriella Magarelli Cínthia Caetano Bonatto Gabriela Mendes da Rocha Vaz Victoria Baggi Mendonça Lauria Luciano Paulino Silva |
| author_sort | Gabriella Magarelli |
| collection | DOAJ |
| description | The global consumption of vegan foods is experiencing an expressive upward trend, underscoring the critical need for quality control measures based on nutritional and functional considerations. This study aimed to evaluate the functional quality of caviar and salmon analog food inks based on pulses combined with nano ingredients and produced in our laboratory (LNANO). The primary objective of this work was to determine the total antioxidant compounds contained in these samples using a voltammetric technique with a glassy carbon electrode. The samples underwent ethanolic extraction (70%) with 1 h of stirring. The voltammograms were acquired in a phosphate buffer electrolyte, pH 3.0 with Ag/AgCl (KCl 3 mol L<sup>−1</sup>) as the reference electrode and platinum wire as the auxiliary electrode. The voltammograms revealed prominent anodic current peaks at 0.76–0.78 V, which are attributed to isoflavones. Isoflavones, known secondary metabolites with substantial antioxidant potential commonly found in pulses, were identified. The total isoflavone concentrations obtained ranged from 31.5 to 64.3 mg Eq genistein 100 g<sup>−1</sup>. The results not only validated the efficacy of the electrochemical sensor for quantifying total antioxidant compounds in the samples but also demonstrated that the concentration of total isoflavones in caviar and salmon analogs fell within the expected limits. |
| first_indexed | 2024-03-11T01:14:38Z |
| format | Article |
| id | doaj.art-6c0cb64b24074d12ba6a3e86437eb5f7 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T01:14:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6c0cb64b24074d12ba6a3e86437eb5f72023-11-18T18:33:30ZengMDPI AGBiosensors2079-63742023-07-0113775110.3390/bios13070751Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor AnalysisGabriella Magarelli0Cínthia Caetano Bonatto1Gabriela Mendes da Rocha Vaz2Victoria Baggi Mendonça Lauria3Luciano Paulino Silva4Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Tecnologias para a Segurança Alimentar (LSA), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Laboratório de Nanobiotecnologia (LNANO), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Laboratório de Nanobiotecnologia (LNANO), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Laboratório de Nanobiotecnologia (LNANO), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Laboratório de Nanobiotecnologia (LNANO), Parque Estação Biológica, Final W5 Norte, Brasília 70770-917, DF, BrazilThe global consumption of vegan foods is experiencing an expressive upward trend, underscoring the critical need for quality control measures based on nutritional and functional considerations. This study aimed to evaluate the functional quality of caviar and salmon analog food inks based on pulses combined with nano ingredients and produced in our laboratory (LNANO). The primary objective of this work was to determine the total antioxidant compounds contained in these samples using a voltammetric technique with a glassy carbon electrode. The samples underwent ethanolic extraction (70%) with 1 h of stirring. The voltammograms were acquired in a phosphate buffer electrolyte, pH 3.0 with Ag/AgCl (KCl 3 mol L<sup>−1</sup>) as the reference electrode and platinum wire as the auxiliary electrode. The voltammograms revealed prominent anodic current peaks at 0.76–0.78 V, which are attributed to isoflavones. Isoflavones, known secondary metabolites with substantial antioxidant potential commonly found in pulses, were identified. The total isoflavone concentrations obtained ranged from 31.5 to 64.3 mg Eq genistein 100 g<sup>−1</sup>. The results not only validated the efficacy of the electrochemical sensor for quantifying total antioxidant compounds in the samples but also demonstrated that the concentration of total isoflavones in caviar and salmon analogs fell within the expected limits.https://www.mdpi.com/2079-6374/13/7/751salmon analogcaviar analogplant-based foodsvoltammetryglassy carbon electrodeantioxidant capacity |
| spellingShingle | Gabriella Magarelli Cínthia Caetano Bonatto Gabriela Mendes da Rocha Vaz Victoria Baggi Mendonça Lauria Luciano Paulino Silva Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis Biosensors salmon analog caviar analog plant-based foods voltammetry glassy carbon electrode antioxidant capacity |
| title | Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis |
| title_full | Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis |
| title_fullStr | Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis |
| title_full_unstemmed | Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis |
| title_short | Unveiling the Remarkable Antioxidant Activity of Plant-Based Fish and Seafood Analogs through Electrochemical Sensor Analysis |
| title_sort | unveiling the remarkable antioxidant activity of plant based fish and seafood analogs through electrochemical sensor analysis |
| topic | salmon analog caviar analog plant-based foods voltammetry glassy carbon electrode antioxidant capacity |
| url | https://www.mdpi.com/2079-6374/13/7/751 |
| work_keys_str_mv | AT gabriellamagarelli unveilingtheremarkableantioxidantactivityofplantbasedfishandseafoodanalogsthroughelectrochemicalsensoranalysis AT cinthiacaetanobonatto unveilingtheremarkableantioxidantactivityofplantbasedfishandseafoodanalogsthroughelectrochemicalsensoranalysis AT gabrielamendesdarochavaz unveilingtheremarkableantioxidantactivityofplantbasedfishandseafoodanalogsthroughelectrochemicalsensoranalysis AT victoriabaggimendoncalauria unveilingtheremarkableantioxidantactivityofplantbasedfishandseafoodanalogsthroughelectrochemicalsensoranalysis AT lucianopaulinosilva unveilingtheremarkableantioxidantactivityofplantbasedfishandseafoodanalogsthroughelectrochemicalsensoranalysis |