PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila.
Drosophila cryptochrome (CRY) is a key circadian photoreceptor that interacts with the period and timeless proteins (PER and TIM) in a light-dependent manner. We show here that a heat pulse also mediates this interaction, and heat-induced phase shifts are severely reduced in the cryptochrome loss-of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-06-01
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| Series: | PLoS Biology |
| Online Access: | http://europepmc.org/articles/PMC1877818?pdf=render |
| _version_ | 1818620255889522688 |
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| author | Rachna Kaushik Pipat Nawathean Ania Busza Alejandro Murad Patrick Emery Michael Rosbash |
| author_facet | Rachna Kaushik Pipat Nawathean Ania Busza Alejandro Murad Patrick Emery Michael Rosbash |
| author_sort | Rachna Kaushik |
| collection | DOAJ |
| description | Drosophila cryptochrome (CRY) is a key circadian photoreceptor that interacts with the period and timeless proteins (PER and TIM) in a light-dependent manner. We show here that a heat pulse also mediates this interaction, and heat-induced phase shifts are severely reduced in the cryptochrome loss-of-function mutant cry(b). The period mutant per(L) manifests a comparable CRY dependence and dramatically enhanced temperature sensitivity of biochemical interactions and behavioral phase shifting. Remarkably, CRY is also critical for most of the abnormal temperature compensation of per(L) flies, because a per(L); cry(b) strain manifests nearly normal temperature compensation. Finally, light and temperature act together to affect rhythms in wild-type flies. The results indicate a role for CRY in circadian temperature as well as light regulation and suggest that these two features of the external 24-h cycle normally act together to dictate circadian phase. |
| first_indexed | 2024-12-16T17:50:29Z |
| format | Article |
| id | doaj.art-6c53c2f2fbaa4860924cc4b6aad3652a |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-16T17:50:29Z |
| publishDate | 2007-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-6c53c2f2fbaa4860924cc4b6aad3652a2022-12-21T22:22:20ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-06-0156e14610.1371/journal.pbio.0050146PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila.Rachna KaushikPipat NawatheanAnia BuszaAlejandro MuradPatrick EmeryMichael RosbashDrosophila cryptochrome (CRY) is a key circadian photoreceptor that interacts with the period and timeless proteins (PER and TIM) in a light-dependent manner. We show here that a heat pulse also mediates this interaction, and heat-induced phase shifts are severely reduced in the cryptochrome loss-of-function mutant cry(b). The period mutant per(L) manifests a comparable CRY dependence and dramatically enhanced temperature sensitivity of biochemical interactions and behavioral phase shifting. Remarkably, CRY is also critical for most of the abnormal temperature compensation of per(L) flies, because a per(L); cry(b) strain manifests nearly normal temperature compensation. Finally, light and temperature act together to affect rhythms in wild-type flies. The results indicate a role for CRY in circadian temperature as well as light regulation and suggest that these two features of the external 24-h cycle normally act together to dictate circadian phase.http://europepmc.org/articles/PMC1877818?pdf=render |
| spellingShingle | Rachna Kaushik Pipat Nawathean Ania Busza Alejandro Murad Patrick Emery Michael Rosbash PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. PLoS Biology |
| title | PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. |
| title_full | PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. |
| title_fullStr | PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. |
| title_full_unstemmed | PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. |
| title_short | PER-TIM interactions with the photoreceptor cryptochrome mediate circadian temperature responses in Drosophila. |
| title_sort | per tim interactions with the photoreceptor cryptochrome mediate circadian temperature responses in drosophila |
| url | http://europepmc.org/articles/PMC1877818?pdf=render |
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