Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats
Noscapine hydrochloride (benzyl-isoquinoline antitussive alkaloid) is an opium derivative and generally used as a cough suppressant. Numerous studies on noscapine hydrochloride have reported that it has potent anti-inflammatory activity. However, the mechanisms by which it exerts an anti-inflammator...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016422002560 |
| _version_ | 1797978763757092864 |
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| author | Shanila Akhter Hafiz Muhammad Irfan Alamgeer Aman Ullah Shah Jahan Muhammad Roman Muhammad Bilal Latif Zaid Mustafa Farooq M. Almutairi Yusuf S. Althobaiti |
| author_facet | Shanila Akhter Hafiz Muhammad Irfan Alamgeer Aman Ullah Shah Jahan Muhammad Roman Muhammad Bilal Latif Zaid Mustafa Farooq M. Almutairi Yusuf S. Althobaiti |
| author_sort | Shanila Akhter |
| collection | DOAJ |
| description | Noscapine hydrochloride (benzyl-isoquinoline antitussive alkaloid) is an opium derivative and generally used as a cough suppressant. Numerous studies on noscapine hydrochloride have reported that it has potent anti-inflammatory activity. However, the mechanisms by which it exerts an anti-inflammatory function is not well understood. Protein denaturation is the primary step that leads to the organ destruction and permanent arthritic disability. The above-mentioned facts provided the ground to plan this study using different in-vitro and in-vivo approaches. RT-qPCR and ELISA assays were used to assess the inflammatory markers related to protein denaturation in complete adjuvant persuaded rheumatism in Sprague - Dawley rats. The results were collected as paw volume and body weight changes, arthritic scoring and serum antioxidant enzymes assays. These findings demonstrated that all doses of noscapine hydrochloride (10, 20 and 40 mg/kg) studied in this study, significantly (p < 0.001) decreased the protein denaturation by preventing the increase in levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nuclear factor-kB (NF-kB), cyclooxygenase-2 (COX-2) and prostaglandin E2. Noscapine hydrochloride significantly reduced the paw volume (p < 0.001), arthritic scoring and reversed the body mass as compared to arthritic control diseased rats. |
| first_indexed | 2024-04-11T05:28:11Z |
| format | Article |
| id | doaj.art-6c56fc32437f4ca08464aefd3b29ccb1 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-11T05:28:11Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-6c56fc32437f4ca08464aefd3b29ccb12022-12-23T04:40:01ZengElsevierSaudi Pharmaceutical Journal1319-01642022-12-01301217911801Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic ratsShanila Akhter0Hafiz Muhammad Irfan1 Alamgeer2Aman Ullah3Shah Jahan4Muhammad Roman5Muhammad Bilal Latif6Zaid Mustafa7Farooq M. Almutairi8Yusuf S. Althobaiti9College of Pharmacy, University of Sargodha, Sargodha, 40100, PakistanCollege of Pharmacy, University of Sargodha, Sargodha, 40100, Pakistan; Corresponding authors at: Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, University of Hafr AlBatin, Hafr AlBatin 39524, Eastern Provine, Saudi Arabia. (F.M. Almutairi).Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore, 54590, PakistanCollege of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad, 44000, PakistanDepartment of Immunology, University of Health Sciences, Lahore, 54600, PakistanDepartment of Immunology, University of Health Sciences, Lahore, 54600, PakistanDepartment of Pathology and Laboratory Medicine, School of Medicine, Emory University, Atlanta, 30322, USADepartment of Agricultural Sciences, Allama Iqbal Open University Islamabad, 44000, PakistanDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, University of Hafr AlBatin, Hafr AlBatin, 39524, Saudi Arabia; Corresponding authors at: Department of Clinical Laboratories Sciences, College of Applied Medical Sciences, University of Hafr AlBatin, Hafr AlBatin 39524, Eastern Provine, Saudi Arabia. (F.M. Almutairi).Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, 21944, Saudi Arabia; Addiction and Neuroscience Research Unit, Taif University, 21944, Saudi ArabiaNoscapine hydrochloride (benzyl-isoquinoline antitussive alkaloid) is an opium derivative and generally used as a cough suppressant. Numerous studies on noscapine hydrochloride have reported that it has potent anti-inflammatory activity. However, the mechanisms by which it exerts an anti-inflammatory function is not well understood. Protein denaturation is the primary step that leads to the organ destruction and permanent arthritic disability. The above-mentioned facts provided the ground to plan this study using different in-vitro and in-vivo approaches. RT-qPCR and ELISA assays were used to assess the inflammatory markers related to protein denaturation in complete adjuvant persuaded rheumatism in Sprague - Dawley rats. The results were collected as paw volume and body weight changes, arthritic scoring and serum antioxidant enzymes assays. These findings demonstrated that all doses of noscapine hydrochloride (10, 20 and 40 mg/kg) studied in this study, significantly (p < 0.001) decreased the protein denaturation by preventing the increase in levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nuclear factor-kB (NF-kB), cyclooxygenase-2 (COX-2) and prostaglandin E2. Noscapine hydrochloride significantly reduced the paw volume (p < 0.001), arthritic scoring and reversed the body mass as compared to arthritic control diseased rats.http://www.sciencedirect.com/science/article/pii/S1319016422002560Benzylisoquinoline alkaloidsProtein denaturationPro- and anti-inflammatory cytokinesAntioxidant |
| spellingShingle | Shanila Akhter Hafiz Muhammad Irfan Alamgeer Aman Ullah Shah Jahan Muhammad Roman Muhammad Bilal Latif Zaid Mustafa Farooq M. Almutairi Yusuf S. Althobaiti Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats Saudi Pharmaceutical Journal Benzylisoquinoline alkaloids Protein denaturation Pro- and anti-inflammatory cytokines Antioxidant |
| title | Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats |
| title_full | Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats |
| title_fullStr | Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats |
| title_full_unstemmed | Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats |
| title_short | Noscapine hydrochloride (benzyl-isoquinoline alkaloid) effectively prevents protein denaturation through reduction of IL-6, NF-kB, COX-2, Prostaglandin-E2 in rheumatic rats |
| title_sort | noscapine hydrochloride benzyl isoquinoline alkaloid effectively prevents protein denaturation through reduction of il 6 nf kb cox 2 prostaglandin e2 in rheumatic rats |
| topic | Benzylisoquinoline alkaloids Protein denaturation Pro- and anti-inflammatory cytokines Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S1319016422002560 |
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