High throughput DNA extraction of legume root nodules for rhizobial metagenomics

High throughput DNA extraction of legume root nodules for rhizobial metagenomics

Abstract A novel combination of tissue homogenization, cell lysis, and DNA purification techniques was developed for isolating total DNA from whole legume root nodules on an automated robotic system. Silica dehydrated root nodules from soy bean, Glycine max cv. Tara, and mung bean, Vigna radiata cv....

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Bibliographic Details
Main Author: Gabriel Johnson
Format: Article
Language:English
Published: SpringerOpen 2019-04-01
Series:AMB Express
Subjects:
Legume
Root nodule
High-throughput
DNA extraction
Rhizobia
Online Access:http://link.springer.com/article/10.1186/s13568-019-0771-z

Internet

http://link.springer.com/article/10.1186/s13568-019-0771-z

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