In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds
Sweet orange (SO) (Citrus sinensis) is a rich source of polyphenols. However, the bioactivity depends directly on the bioaccessibility affected by biological factors during the digestion process. Therefore, this study was performed in lyophilized SO samples before and after an in vitro digestion to...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621005405 |
| _version_ | 1831756833281277952 |
|---|---|
| author | Gloria Itzel Peña-Vázquez Maria Teresa Dominguez-Fernández Bianka Dianey Camacho-Zamora Marcelo Hernandez-Salazar Vania Urías-Orona María-Paz De Peña Ana Laura de la Garza |
| author_facet | Gloria Itzel Peña-Vázquez Maria Teresa Dominguez-Fernández Bianka Dianey Camacho-Zamora Marcelo Hernandez-Salazar Vania Urías-Orona María-Paz De Peña Ana Laura de la Garza |
| author_sort | Gloria Itzel Peña-Vázquez |
| collection | DOAJ |
| description | Sweet orange (SO) (Citrus sinensis) is a rich source of polyphenols. However, the bioactivity depends directly on the bioaccessibility affected by biological factors during the digestion process. Therefore, this study was performed in lyophilized SO samples before and after an in vitro digestion to obtain total phenolic content (TPC) by Folin-Ciocalteu assay and characterize its phenolic profile by HPLC-ESI-MS/MS with methanolic extracts. Simulating the passive diffusion of biocompounds, samples were dialyzed after intestinal digestion to obtain bioaccessible phenolic compounds (BPC). Ethanolic extracts were used in 3T3-L1 cell culture. The in vitro digestion process decreased 25% the bioaccessibility of SO polyphenols in the intestinal phase. The most abundant subgroup of polyphenols before and after in vitro digestion were the flavanones, representing more than 86% of TPC. Finally, lipid accumulation in cells induced to undergo adipogenesis and treated with BPC decreased although without significant differences at different concentrations. |
| first_indexed | 2024-12-22T00:20:54Z |
| format | Article |
| id | doaj.art-6c66e5dd0b974180a6eea23fe2f4ff1d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-22T00:20:54Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6c66e5dd0b974180a6eea23fe2f4ff1d2022-12-21T18:45:10ZengElsevierJournal of Functional Foods1756-46462022-01-0188104891In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compoundsGloria Itzel Peña-Vázquez0Maria Teresa Dominguez-Fernández1Bianka Dianey Camacho-Zamora2Marcelo Hernandez-Salazar3Vania Urías-Orona4María-Paz De Peña5Ana Laura de la Garza6Universidad Autonoma de Nuevo Leon, Facultad de Salud Pública y Nutrición, Centro de Investigación en Nutrición y Salud Pública, Monterrey, Nuevo León, MexicoUniversidad de Navarra, Facultad de Farmacia y Nutrición, Departamento de Ciencias de la Alimentación y Fisiología, C/Irunlarrea 1, E-31008 Pamplona, SpainUniversidad Autonoma de Nuevo Leon, Unidad de Genómica, Centro de Investigación y Desarrollo en Ciencias de la Salud, Monterrey, Nuevo León, MexicoUniversidad Autonoma de Nuevo Leon, Facultad de Salud Pública y Nutrición, Centro de Investigación en Nutrición y Salud Pública, Monterrey, Nuevo León, MexicoUniversidad Autonoma de Nuevo Leon, Facultad de Salud Pública y Nutrición, Centro de Investigación en Nutrición y Salud Pública, Monterrey, Nuevo León, MexicoUniversidad de Navarra, Facultad de Farmacia y Nutrición, Departamento de Ciencias de la Alimentación y Fisiología, C/Irunlarrea 1, E-31008 Pamplona, SpainUniversidad Autonoma de Nuevo Leon, Facultad de Salud Pública y Nutrición, Centro de Investigación en Nutrición y Salud Pública, Monterrey, Nuevo León, Mexico; Universidad Autonoma de Nuevo Leon, Unidad de Nutrición, Centro de Investigación y Desarrollo en Ciencias de la Salud, Monterrey, Nuevo León, Mexico; Corresponding author at: Universidad Autónoma de Nuevo León, Facultad de Salud Pública y Nutrición, Centro de Investigación en Nutrición y Salud Pública, Mexico.Sweet orange (SO) (Citrus sinensis) is a rich source of polyphenols. However, the bioactivity depends directly on the bioaccessibility affected by biological factors during the digestion process. Therefore, this study was performed in lyophilized SO samples before and after an in vitro digestion to obtain total phenolic content (TPC) by Folin-Ciocalteu assay and characterize its phenolic profile by HPLC-ESI-MS/MS with methanolic extracts. Simulating the passive diffusion of biocompounds, samples were dialyzed after intestinal digestion to obtain bioaccessible phenolic compounds (BPC). Ethanolic extracts were used in 3T3-L1 cell culture. The in vitro digestion process decreased 25% the bioaccessibility of SO polyphenols in the intestinal phase. The most abundant subgroup of polyphenols before and after in vitro digestion were the flavanones, representing more than 86% of TPC. Finally, lipid accumulation in cells induced to undergo adipogenesis and treated with BPC decreased although without significant differences at different concentrations.http://www.sciencedirect.com/science/article/pii/S1756464621005405CitrusFlavanonesBioaccessibilityAdipogenesisLipolysisObesity |
| spellingShingle | Gloria Itzel Peña-Vázquez Maria Teresa Dominguez-Fernández Bianka Dianey Camacho-Zamora Marcelo Hernandez-Salazar Vania Urías-Orona María-Paz De Peña Ana Laura de la Garza In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds Journal of Functional Foods Citrus Flavanones Bioaccessibility Adipogenesis Lipolysis Obesity |
| title | In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds |
| title_full | In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds |
| title_fullStr | In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds |
| title_full_unstemmed | In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds |
| title_short | In vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of Sweet orange (Citrus sinensis) phenolic compounds |
| title_sort | in vitro simulated gastrointestinal digestion impacts bioaccessibility and bioactivity of sweet orange citrus sinensis phenolic compounds |
| topic | Citrus Flavanones Bioaccessibility Adipogenesis Lipolysis Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621005405 |
| work_keys_str_mv | AT gloriaitzelpenavazquez invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT mariateresadominguezfernandez invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT biankadianeycamachozamora invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT marcelohernandezsalazar invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT vaniauriasorona invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT mariapazdepena invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds AT analauradelagarza invitrosimulatedgastrointestinaldigestionimpactsbioaccessibilityandbioactivityofsweetorangecitrussinensisphenoliccompounds |