Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.

Alterations in bone remodeling are a major public health issue, as therapeutic options for widespread bone disorders such as osteoporosis and tumor-induced osteolysis are still limited. Therefore, a detailed understanding of the regulatory mechanism governing bone cell differentiation in health and...

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Main Authors: Timo Heckt, Thomas Bickert, Anke Jeschke, Sebastian Seitz, Jochen Schulze, Wulf D Ito, Wolfgang Zimmermann, Michael Amling, Thorsten Schinke, Andrea Kristina Horst, Johannes Keller
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4260834?pdf=render
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author Timo Heckt
Thomas Bickert
Anke Jeschke
Sebastian Seitz
Jochen Schulze
Wulf D Ito
Wolfgang Zimmermann
Michael Amling
Thorsten Schinke
Andrea Kristina Horst
Johannes Keller
author_facet Timo Heckt
Thomas Bickert
Anke Jeschke
Sebastian Seitz
Jochen Schulze
Wulf D Ito
Wolfgang Zimmermann
Michael Amling
Thorsten Schinke
Andrea Kristina Horst
Johannes Keller
author_sort Timo Heckt
collection DOAJ
description Alterations in bone remodeling are a major public health issue, as therapeutic options for widespread bone disorders such as osteoporosis and tumor-induced osteolysis are still limited. Therefore, a detailed understanding of the regulatory mechanism governing bone cell differentiation in health and disease are of utmost clinical importance. Here we report a novel function of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), a member of the immunoglobulin superfamily involved in inflammation and tumorigenesis, in the physiologic regulation of bone remodeling. Assessing the expression of all members of the murine Ceacam family in bone tissue and marrow, we found CEACAM1 and CEACAM10 to be differentially expressed in both bone-forming osteoblasts and bone-resorbing osteoclasts. While Ceacam10-deficient mice displayed no alteration in structural bone parameters, static histomorphometry demonstrated a reduced trabecular bone mass in mice lacking CEACAM1. Furthermore, cellular and dynamic histomorphometry revealed an increased osteoclast formation in Ceacam1-deficient mice, while osteoblast parameters and the bone formation rate remained unchanged. In line with these findings, we detected accelerated osteoclastogenesis in Ceacam1-deficient bone marrow cells, while osteoblast differentiation, as determined by mineralization and alkaline phosphatase assays, was not affected. Therefore, our results provide in vivo and in vitro evidence for a physiologic role of CEACAM1 in the regulation of osteoclastogenesis.
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spelling doaj.art-6c838fd5aa3346c5ac7ac08bd25aecb92022-12-22T01:23:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11436010.1371/journal.pone.0114360Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.Timo HecktThomas BickertAnke JeschkeSebastian SeitzJochen SchulzeWulf D ItoWolfgang ZimmermannMichael AmlingThorsten SchinkeAndrea Kristina HorstJohannes KellerAlterations in bone remodeling are a major public health issue, as therapeutic options for widespread bone disorders such as osteoporosis and tumor-induced osteolysis are still limited. Therefore, a detailed understanding of the regulatory mechanism governing bone cell differentiation in health and disease are of utmost clinical importance. Here we report a novel function of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), a member of the immunoglobulin superfamily involved in inflammation and tumorigenesis, in the physiologic regulation of bone remodeling. Assessing the expression of all members of the murine Ceacam family in bone tissue and marrow, we found CEACAM1 and CEACAM10 to be differentially expressed in both bone-forming osteoblasts and bone-resorbing osteoclasts. While Ceacam10-deficient mice displayed no alteration in structural bone parameters, static histomorphometry demonstrated a reduced trabecular bone mass in mice lacking CEACAM1. Furthermore, cellular and dynamic histomorphometry revealed an increased osteoclast formation in Ceacam1-deficient mice, while osteoblast parameters and the bone formation rate remained unchanged. In line with these findings, we detected accelerated osteoclastogenesis in Ceacam1-deficient bone marrow cells, while osteoblast differentiation, as determined by mineralization and alkaline phosphatase assays, was not affected. Therefore, our results provide in vivo and in vitro evidence for a physiologic role of CEACAM1 in the regulation of osteoclastogenesis.http://europepmc.org/articles/PMC4260834?pdf=render
spellingShingle Timo Heckt
Thomas Bickert
Anke Jeschke
Sebastian Seitz
Jochen Schulze
Wulf D Ito
Wolfgang Zimmermann
Michael Amling
Thorsten Schinke
Andrea Kristina Horst
Johannes Keller
Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
PLoS ONE
title Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
title_full Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
title_fullStr Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
title_full_unstemmed Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
title_short Increased osteoclastogenesis in mice lacking the carcinoembryonic antigen-related cell adhesion molecule 1.
title_sort increased osteoclastogenesis in mice lacking the carcinoembryonic antigen related cell adhesion molecule 1
url http://europepmc.org/articles/PMC4260834?pdf=render
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