Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms. Similarly, natural or designed proteins are mutagenized at targeted...

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Bibliographic Details
Main Authors: Herbert M. Huttanus, Ellin-Kristina H. Triola, Jeanette C. Velasquez-Guzman, Sang-Min Shin, Rommel S. Granja-Travez, Anmoldeep Singh, Taraka Dale, Ramesh K. Jha
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-07-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
protein engineering
synthetic biology
promoter engineering
mutagenesis
polymerase chain reaction
overlap extension PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202388/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202388/full

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