Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood
A two-step process using colloid milling (CM) and hot water (HW) treatment was evaluated for its ability to improve xylose recovery and the enzymatic digestibility of oak wood. In the first step, CM pretreatment was applied at a milling (feeding) speed of 100 mL/min with four different milling times...
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MDPI AG
2022-03-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/15/6/2210 |
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| author | Tae Hoon Kim Seung Hyeon Park Tin Diep Trung Le Tae Hyun Kim Kyeong Keun Oh |
| author_facet | Tae Hoon Kim Seung Hyeon Park Tin Diep Trung Le Tae Hyun Kim Kyeong Keun Oh |
| author_sort | Tae Hoon Kim |
| collection | DOAJ |
| description | A two-step process using colloid milling (CM) and hot water (HW) treatment was evaluated for its ability to improve xylose recovery and the enzymatic digestibility of oak wood. In the first step, CM pretreatment was applied at a milling (feeding) speed of 100 mL/min with four different milling times (3, 6, 9, and 12 h), and the enzymatic digestibility and physical properties of each substrate were measured. In the second-step, the HW pretreatment was applied to enhance the enzymatic digestibility and xylan recovery at various reaction severities (<i>Log R</i><sub>0</sub>) from 2.07 to 4.43 using 12 h colloid-milled (CM-treated) oak wood. Compared with untreated oak wood, CM not only significantly disrupted the structure of oak wood but also increased its Brunauer–Emmett–Teller surface area (42-fold) and pore volume (28-fold). The crystallinity of two-step-treated oak wood was decreased to 34.8, while the enzymatic digestibility of 12 h CM-treated oak wood was increased to 58.1% at enzyme loading of 30 filter paper units (FPU)/g glucan for 96 h. After HW treatment of CM-treated oak wood at <i>Log R</i><sub>0</sub> = 3.83, 80.7% of xylan recovery yield and 91.1% of enzymatic digestibility (with 15 FPU/g glucan at 96 h) was obtained, which was 84.2% higher than the enzymatic digestibility of untreated oak wood (6.9%). |
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| id | doaj.art-6c95d3cfe5d24a048801375b55cd5723 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T13:43:15Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
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| series | Energies |
| spelling | doaj.art-6c95d3cfe5d24a048801375b55cd57232023-11-30T21:03:36ZengMDPI AGEnergies1996-10732022-03-01156221010.3390/en15062210Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak WoodTae Hoon Kim0Seung Hyeon Park1Tin Diep Trung Le2Tae Hyun Kim3Kyeong Keun Oh4R&D Center, SugarEn Co., Ltd., Yongin 16890, Gyeonggi-do, KoreaDepartment of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Gyeonggi-do, KoreaDepartment of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Gyeonggi-do, KoreaDepartment of Materials Science and Chemical Engineering, Hanyang University, Ansan 15588, Gyeonggi-do, KoreaR&D Center, SugarEn Co., Ltd., Yongin 16890, Gyeonggi-do, KoreaA two-step process using colloid milling (CM) and hot water (HW) treatment was evaluated for its ability to improve xylose recovery and the enzymatic digestibility of oak wood. In the first step, CM pretreatment was applied at a milling (feeding) speed of 100 mL/min with four different milling times (3, 6, 9, and 12 h), and the enzymatic digestibility and physical properties of each substrate were measured. In the second-step, the HW pretreatment was applied to enhance the enzymatic digestibility and xylan recovery at various reaction severities (<i>Log R</i><sub>0</sub>) from 2.07 to 4.43 using 12 h colloid-milled (CM-treated) oak wood. Compared with untreated oak wood, CM not only significantly disrupted the structure of oak wood but also increased its Brunauer–Emmett–Teller surface area (42-fold) and pore volume (28-fold). The crystallinity of two-step-treated oak wood was decreased to 34.8, while the enzymatic digestibility of 12 h CM-treated oak wood was increased to 58.1% at enzyme loading of 30 filter paper units (FPU)/g glucan for 96 h. After HW treatment of CM-treated oak wood at <i>Log R</i><sub>0</sub> = 3.83, 80.7% of xylan recovery yield and 91.1% of enzymatic digestibility (with 15 FPU/g glucan at 96 h) was obtained, which was 84.2% higher than the enzymatic digestibility of untreated oak wood (6.9%).https://www.mdpi.com/1996-1073/15/6/2210fractionationwoody biomasssuper-colloid millhydrothermal pretreatmentmulti-step processing |
| spellingShingle | Tae Hoon Kim Seung Hyeon Park Tin Diep Trung Le Tae Hyun Kim Kyeong Keun Oh Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood Energies fractionation woody biomass super-colloid mill hydrothermal pretreatment multi-step processing |
| title | Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood |
| title_full | Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood |
| title_fullStr | Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood |
| title_full_unstemmed | Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood |
| title_short | Effects of Colloid Milling and Hot-Water Pretreatment on Physical Properties and Enzymatic Digestibility of Oak Wood |
| title_sort | effects of colloid milling and hot water pretreatment on physical properties and enzymatic digestibility of oak wood |
| topic | fractionation woody biomass super-colloid mill hydrothermal pretreatment multi-step processing |
| url | https://www.mdpi.com/1996-1073/15/6/2210 |
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